This study assessed interrater and intrarater reliability of Mohs surgeons in interpreting MART-1 immunostained frozen sections for melanoma margin evaluation. The findings quantified agreement levels and identified areas of interpretive uncertainty, informing quality improvement and training efforts.

Mohs surgeon interpretation of MART-1 frozen sections shows good but imperfect interrater reliability, highlighting the need for standardized training.

Intrarater reliability is generally higher than interrater reliability, suggesting individual consistency but potential for systematic differences between surgeons.