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MohsPedia/Sutures & Closures

Suturing Techniques

A thorough guide to suturing techniques in dermatologic surgery, from the workhorse buried vertical mattress to superficial closure methods. Covers suture placement biomechanics, tissue eversion principles, tension management, and suture removal timing.

By Dr. Yehonatan Kaplan (M.D., Fellow ACMS)·Published: 2025-03-01·Updated: 2026-03-07·Reviewed: 2026-03-07
suture techniqueburied vertical mattresshorizontal mattressrunning subcuticulartip stitcheversionwound closuresuture removal
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The Buried Vertical Mattress (Dermal) Suture

The buried vertical mattress suture is the workhorse of deep closure in dermatologic surgery. It is the most commonly placed suture in Mohs and excisional surgery, responsible for carrying wound tension, everting wound edges, and eliminating dead space. Mastery of this technique is the single most important suturing skill for the dermatologic surgeon.

Geometry: The Heart-Shaped Loop

When placed correctly, the buried vertical mattress suture traces a heart-shaped path through the tissue. The needle enters at the depth of the wound (base of the dermis or deep reticular dermis), curves upward through the mid-dermis (peak), and then descends back to the wound base on the opposite side. The critical geometric feature is that the widest point of the suture loop (the peak) is at the mid-dermis level, while the narrowest point (the valley) is at the wound base. The knot is buried at the deepest point of the loop. At the bottom of the wound. Which is essential for two reasons: it prevents the knot from extruding through the skin surface, and it places the knot away from the healing wound edge.

Step-by-Step Placement

First bite: Enter the deep dermis at the wound base on the far side of the wound, approximately 3-5 mm from the wound edge depending on tissue thickness. Drive the needle upward through the reticular dermis, curving to exit at the mid-papillary dermis level approximately 2-4 mm from the wound edge. The needle tip should exit at the level where the dermis transitions from reticular to papillary. Second bite: On the near side, enter at the corresponding mid-papillary dermis level at the same distance from the wound edge, and drive the needle downward through the dermis to exit at the wound base. Tie the knot at the wound base with the knot positioned deep in the wound.

Suture Size Selection by Tension

Suture size must match the tensile load of the wound. Using a suture that is too small risks suture breakage and wound dehiscence; using a suture that is too large causes unnecessary tissue reaction and may leave visible suture marks if percutaneous.
Suture SizeTension LevelTypical Anatomical SitesNotes
2-0High tensionBack, trunk, scalp, shouldersLargest commonly used in derm surgery; provides maximum strength for high-tension closures
3-0Moderate-to-high tensionExtremities, lateral trunk, posterior neckVersatile workhorse size for trunk and extremities
4-0Moderate tensionFace (most areas), anterior neck, proximal extremitiesMost common deep suture size for facial closures
5-0Minimal tensionEyelids, ears, nasal tip, lip vermilionUsed where tissue is thin and tension is minimal; also for superficial skin sutures on face
6-0Very low tensionEyelid margin, vermilion border, delicate free marginsFinest gauge used in derm surgery; primarily for percutaneous sutures on the most delicate facial subunits

Countertraction and Tissue Handling

Proper countertraction during suture placement is essential for visibility, accuracy, and wound edge eversion. The assistant (or the surgeon using the non-dominant hand) provides countertraction using skin hooks or toothed forceps.

Retraction Level

Retract the wound edge at the papillary dermis level. This is the optimal retraction plane because it simultaneously everts the wound edge (exposing the dermal surface for suture placement) and maximizes visualization of the deep wound architecture. Retracting too superficially (epidermis) inverts the edge; retracting too deeply (subcutis) obscures the dermal anatomy.

The Snap Test

After placing the first bite of a buried vertical mattress suture (before the second bite), perform the snap test: gently release the wound edge and observe whether it snaps toward the center of the wound. If the edge springs medially, the suture bite has adequate purchase in the dermis and will produce good eversion. If the edge does not move or falls laterally, the bite is too superficial or too deep and should be re-placed.

The Pull Test

After completing deep closure, perform the pull test: grasp the wound edges on both sides and pull them apart. Inspect the closure line for any gaps or areas of poor approximation. Each gap indicates a location where an additional deep suture is needed. This simple test prevents the common error of placing too few deep sutures and relying on superficial sutures to carry tension.

Adapting to Tissue Thickness

The geometry of the buried vertical mattress suture must be adjusted based on dermal thickness. Failure to adapt technique to tissue characteristics is a common source of suboptimal closures.

Thick Dermis (Back, Scalp, Trunk)

In thick-skinned areas, the buried vertical mattress should have a wider bite (farther from wound edge), greater peak-to-valley distance (taller suture loop), and a broad, rounded heart shape. The peak of the suture should still reach the papillary dermis, but the overall loop is larger to encompass the thicker dermal collagen. Take bites 4-6 mm from the wound edge.

Thin Dermis (Eyelid, Ear, Nasal Tip)

In thin-skinned areas, the buried vertical mattress requires a narrower bite (closer to wound edge, 2-3 mm), smaller peak-to-valley distance, and a tall, narrow heart shape. The margin for error is much smaller. Bites that are even slightly too wide or too deep will result in suture extrusion or tissue strangulation. Use 5-0 or 6-0 absorbable suture.

Three Suboptimal Suture Paths

Understanding common errors in buried vertical mattress placement helps surgeons self-correct and achieve consistent results.

1. Circular Path (Too Narrow)

When the suture traces a circular rather than heart-shaped path, the peak does not adequately reach the papillary dermis. This results in poor eversion. The wound edges lie flat or even invert. The fix is to drive the needle more superficially at the peak of the loop, ensuring the peak reaches the mid-to-upper papillary dermis.

2. Excessively Wide and Deep Path

When suture bites are too far from the wound edge and too deep, the suture loop encompasses excessive tissue. This creates tissue strangulation, ischemia at the wound edge, and paradoxically poor approximation because the suture cinches tissue too far from the wound margin. The fix is to take bites closer to the wound edge and reduce the depth of the valley.

3. Eversion-Inversion Collapse

When the suture initially everts the wound edge but then collapses into inversion as the knot is tightened, the problem is usually that the peak of the suture loop is at different levels on the two sides of the wound. Asymmetric bites cause one edge to evert while the other inverts, and the net result is a flat or inverted closure. The fix is to ensure symmetric bite depths and peak levels on both sides.

Superficial Suturing Techniques

Superficial (epidermal/percutaneous) sutures approximate the epidermal wound edges and should carry NO tension if deep closure is adequate. Their role is alignment and edge apposition, not load-bearing.

Simple Interrupted

The most basic and versatile superficial suture. The needle enters perpendicular to the skin surface 2-3 mm from the wound edge, passes through the dermis in a flask-shaped (wider at base) or symmetric arc, and exits on the opposite side at the same distance from the edge. For proper eversion, the suture should encompass more tissue at depth than at the surface. Knot is tied with the square knot on one side of the wound (not directly over the incision line).

Height Correction Principle

When wound edges are at different heights (a common occurrence after flap advancement or when tissue thickness differs), the simple interrupted suture can correct the discrepancy. The rule is: "bite high on the high side, low on the low side." On the elevated wound edge, enter the needle farther from the wound edge and more superficially. On the depressed edge, enter closer to the edge and deeper. This differential bite geometry draws the higher edge down and the lower edge up, producing a level closure.

Horizontal Mattress

Two parallel simple suture bites separated by 4-8 mm. Provides excellent eversion and distributes tension over a wider area. Useful for high-tension areas (scalp, trunk) and as an initial anchoring suture at the center of a long wound. Disadvantage: can strangulate tissue between the two bites if tied too tightly, causing necrosis of the skin bridge.

Running Subcuticular

A continuous suture placed horizontally within the mid-dermis, parallel to the skin surface, with entry and exit points at the wound apices. The suture passes back and forth between wound edges in the horizontal plane, advancing 3-5 mm per bite along the wound length. Provides excellent cosmetic results (no cross-hatch marks) and is ideal for long, straight wounds under minimal tension. Commonly placed with polypropylene (removable) or Monocryl (absorbable). Remove polypropylene running subcuticular sutures at 2-3 weeks.

Corner Stitch (Tip Stitch / Half-Buried Horizontal Mattress)

A specialized suture for securing flap tips without compromising blood supply. The needle enters percutaneously through the non-tip side, then passes horizontally through the dermis of the flap tip (buried component), and exits percutaneously on the other non-tip side. Only the tip passes through the dermis in a buried fashion. No suture passes through the tip epidermis, preserving tip vascularity. This is essential for rotation flaps, advancement flaps, and any closure where a triangular tissue tip must be secured.

Suture Removal Timing

Suture removal timing balances two competing forces: early removal reduces suture-track marks (cross-hatching) but risks wound dehiscence; delayed removal improves wound strength but increases scarring from suture marks. Timing depends on anatomical location, wound tension, and patient factors.
LocationRemoval TimingRationale
Face and neck5-7 daysExcellent blood supply provides rapid healing; thin skin shows suture marks quickly
Scalp7-10 days (staples) or 10-14 days (sutures)High tension; thick skin tolerates longer suture retention
Trunk (chest, abdomen, back)7-10 daysModerate tension; slower healing than face
Upper extremities7-10 daysModerate tension and mobility
Lower extremities10-14 daysPoor vascularity, high tension, slow healing. Longest retention
Hands and feet10-14 daysHigh functional stress; slower healing
Over joints10-14 daysConstant motion requires prolonged support

Advanced Techniques and Pearls

Several additional techniques and principles enhance closure quality in dermatologic surgery.

Rule of Halves

For long linear wounds, place the first deep suture at the midpoint, then bisect each remaining segment iteratively. This technique (first suture at 1/2, then 1/4 and 3/4, then 1/8 positions, etc.) ensures symmetric closure and prevents tissue bunching or dog-ear formation at one end.

Instrument Tie vs. Hand Tie

Instrument ties (using needle driver to create loops) are standard in dermatologic surgery. They conserve suture material and allow precise knot placement. Hand ties are reserved for deep body cavities or when instrument access is limited. For buried sutures, use a surgeon's knot (double first throw) to prevent slippage while the second throw is placed, followed by alternating square throws (typically 4-5 total throws for braided sutures, 5-7 for monofilament).

Frequently Asked Questions

Recent Evidence

From the Northwestern Medicine Dermatologic Surgery Journal Club

Deep Suture Spacing - Effect on Wound Closure Outcomes

Eshagh K, Sklar LR, Pourang A, et al. · Dermatologic Surgery (2022)

Healing Time and Pain in Auricular Wounds - Second Intention Analysis

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SIH (Shave-Incision-Healing) vs Second Intention for Auricular Reconstruction

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Running Subcuticular vs Running Horizontal Mattress Suture - Aesthetic RCT

Kwapnoski Z, Doost MS, Vy M, et al. · Dermatologic Surgery (2024)

View all in Journal Club →
References
  1. [1] Suture materials and suturing techniques for cutaneous wound closure. J Cutan Med Surg. . doi:10.1177/1203475415582089
  2. [2] Dermatological surgery: an update on suture materials and techniques. Part 1. Clin Exp Dermatol. . doi:10.1111/ced.14770
  3. [3] Surgery of the Skin: Procedural Dermatology, 3rd Edition. Elsevier. .
  4. [4] Surgical technique for optimal outcomes: Part I. Cutting, handling, and suturing tissue. J Am Acad Dermatol. . doi:10.1016/j.jaad.2015.02.1143
  5. [5] Surgical technique for optimal outcomes: Part II. Principles of tissue repair. J Am Acad Dermatol. . doi:10.1016/j.jaad.2015.03.033

About This Article

Author: , Fellow ACMS

Last Medical Review:

Audience: Dermatologic Surgeons

Clinic: Kaplan Clinic · DermUnbound Research Program